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pcmv6 entry plasmid  (OriGene)


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    Structured Review

    OriGene pcmv6 entry plasmid
    Pcmv6 Entry Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1675 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv6+entry/pCMV6-Entry+Mammalian+Expression+Vector/pmc13217847-87-0-3
    Average 96 stars, based on 1675 article reviews
    pcmv6 entry plasmid - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: IFI16 senses and protects stalled replication forks.
    Article Snippet: Images were captured and analysed using Zen software (Zeiss) and ImageJ2/FIJI.

    Article Title: Antibody-pyrrolobenzodiazepine derivative conjugate which binds caludin-6 and claudin-9
    Article Snippet: 138-1: Construction of Heavy Chain Expression Vector for Trastuzumab-LALA The DNA fragment consisting of nucleotide residues 36 to 434 of the nucleotide sequence of SEQ ID NO: 74 for the heavy chain of trastuzumab-LALA was synthesized (GeneArt).

    Control:

    Article Title: Mechanistic of LRRK2-Mediated Pyroptosis Via the NLRP3/Caspase-1/GSDMD Pathway in Parkinson’s Disease Progression
    Article Snippet: .. Empty vectors, including pCMV6-Entry and pGFP-C-shLenti (OriGene Technologies, Rockville, MD, USA), were used as control plasmids. .. Total RNA was isolated from HMC3 cells, SH-SY5Y cells, and mouse midbrain tissues across the indicated treatment groups using the EZ-press RNA Purification Kit (EZBioscience, USA).

    Article Title: Squamous cell carcinoma antigen-1/SerpinB3 is an endogenous skin injury response element
    Article Snippet: .. The day after seeding, cells were transfected with pCMV6-Entry containing a c-terminal Myc-DDK tag under control of a CMV promoter (Origene #RC202683) using Attractene reagent (Qiagen) according to manufacturer’s procedure and incubated for 24 hours prior to immunofluorescence. ..

    Cell Culture:

    Article Title: Combination of antibody-pyrrolobenzodiazepine derivative conjugate and PARP inhibitor
    Article Snippet: .. Human CLDN3/pCMV6-Entry, human CLDN4/pCMV6-Entry, human CLDN6/pCMV-Entry, human CLDN9/pCMV6-Entry, or pCMV6-Entry purchased from OriGene Technologies, Inc. was transiently transferred into 293T cells (Thermo Fisher Scientific, HCL4517) by using Lipofectamine 2000 (Thermo Fisher Scientific), and the cells were cultured under conditions of 37° C. and 5% CO2 overnight, and then a cell suspension was prepared. .. The transfected 293T cell suspension was centrifuged to remove the supernatant, and a mouse anti-CLDN6 antibody (clone number: B1 or C7) or a mouse IgG1 control antibody (R&D Systems, Inc.) was then added and suspended to a final concentration of 30 μg/mL, 10 μg/mL, 3.3 μg/mL, or 1.1 μg/mL, and the resultant was left to stand at 4° C. for 1 hour.

    Suspension:

    Article Title: Combination of antibody-pyrrolobenzodiazepine derivative conjugate and PARP inhibitor
    Article Snippet: .. Human CLDN3/pCMV6-Entry, human CLDN4/pCMV6-Entry, human CLDN6/pCMV-Entry, human CLDN9/pCMV6-Entry, or pCMV6-Entry purchased from OriGene Technologies, Inc. was transiently transferred into 293T cells (Thermo Fisher Scientific, HCL4517) by using Lipofectamine 2000 (Thermo Fisher Scientific), and the cells were cultured under conditions of 37° C. and 5% CO2 overnight, and then a cell suspension was prepared. .. The transfected 293T cell suspension was centrifuged to remove the supernatant, and a mouse anti-CLDN6 antibody (clone number: B1 or C7) or a mouse IgG1 control antibody (R&D Systems, Inc.) was then added and suspended to a final concentration of 30 μg/mL, 10 μg/mL, 3.3 μg/mL, or 1.1 μg/mL, and the resultant was left to stand at 4° C. for 1 hour.

    Transfection:

    Article Title: Squamous cell carcinoma antigen-1/SerpinB3 is an endogenous skin injury response element
    Article Snippet: .. The day after seeding, cells were transfected with pCMV6-Entry containing a c-terminal Myc-DDK tag under control of a CMV promoter (Origene #RC202683) using Attractene reagent (Qiagen) according to manufacturer’s procedure and incubated for 24 hours prior to immunofluorescence. ..

    Article Title: Partial Bypass of Frataxin Deficiency by ISCU M141I Restores Cytosolic and Nuclear Fe–S Cluster Assembly
    Article Snippet: .. The background levels corresponding to 55 Fe measurements on eluates after anti-FLAG immunoprecipitations on cytosolic extracts isolated from cells transfected with the empty vector, pCMV6-Entry (Origene Technologies), were also included to account for nonspecific 55 Fe amounts stochastically associated to the beads. .. In parallel, 10 μL of eluates were run on 4-16% native PAGE gels (ThermoFisher), resolved after run at 150V for 1 hour, followed by 2 hours at 250V, and imaged by autoradiogram.

    Incubation:

    Article Title: Squamous cell carcinoma antigen-1/SerpinB3 is an endogenous skin injury response element
    Article Snippet: .. The day after seeding, cells were transfected with pCMV6-Entry containing a c-terminal Myc-DDK tag under control of a CMV promoter (Origene #RC202683) using Attractene reagent (Qiagen) according to manufacturer’s procedure and incubated for 24 hours prior to immunofluorescence. ..

    Immunofluorescence:

    Article Title: Squamous cell carcinoma antigen-1/SerpinB3 is an endogenous skin injury response element
    Article Snippet: .. The day after seeding, cells were transfected with pCMV6-Entry containing a c-terminal Myc-DDK tag under control of a CMV promoter (Origene #RC202683) using Attractene reagent (Qiagen) according to manufacturer’s procedure and incubated for 24 hours prior to immunofluorescence. ..

    Immunocytochemistry:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Flow Cytometry:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Western Blot:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Expressing:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Construct:

    Article Title: Albumin fusion of the rVWF fragment rD'D3 reduces internalisation by clearance receptor Stabilin-2.
    Article Snippet: A recombinant VWF D’D3 albumin fusion protein (rD'D3-FP) has been developed to extend the half-life of coadministered coagulation factor VIII (FVIII) for the treatment of haemophilia A.. We examined the impact of albumin fusion of rD'D3 on the Stabilin-2 (STAB2) clearance receptor pathway and rD'D3-FP recycling via engagement of the neonatal Fc receptor FcRn as two potential mechanisms for rD'D3-FP half-life extension.. Binding and intracellular trafficking of fluorescently labelled rD'D3-FP was examined using human 293-F cells overexpressing STAB2 or in combination with FcRn by flow cytometry and confocal microscopy.

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Isolation:

    Article Title: Partial Bypass of Frataxin Deficiency by ISCU M141I Restores Cytosolic and Nuclear Fe–S Cluster Assembly
    Article Snippet: .. The background levels corresponding to 55 Fe measurements on eluates after anti-FLAG immunoprecipitations on cytosolic extracts isolated from cells transfected with the empty vector, pCMV6-Entry (Origene Technologies), were also included to account for nonspecific 55 Fe amounts stochastically associated to the beads. .. In parallel, 10 μL of eluates were run on 4-16% native PAGE gels (ThermoFisher), resolved after run at 150V for 1 hour, followed by 2 hours at 250V, and imaged by autoradiogram.

    Plasmid Preparation:

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Amplification:

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Clone Assay:

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..

    Cloning:

    Article Title: Dual-site phosphorylation of SLX4 stabilizes the SLX4–MUS81 interface to promote mitotic SMX assembly and genome protection
    Article Snippet: .. To construct plasmid pCMV6 Venus-3XFLAG-SLX4, Venus-3XFLAG-SLX4resist M1A was amplified with primers HDW0910 and HDW0911 and cloned into the SalI and PmeI sites of pCMV6-Entry (Origene) using seamless cloning. ..



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